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For the release of O-linked glycans from glycoprotein therapeutics. The Ludger Liberate Orela Kit has been developed for O-linked deglycosylation of glycoproteins. Released glycans have free-reducing termini to allow fluorescent tagging by reductive amination. The kit includes reagents and materials for O-linked deglycosylation of up to 12 glycoprotein samples analysed in parallel or two sets of 6 samples, typically up to 1 mg of glycoprotein per sample. This method of O-glycan release demonstrates a significant reduction in peeling and reduces the number of sample handling steps before release.
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1,2-O-Isopropylidene-β-L-idofuranuronic-6-13C acid γ-lactone
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1,2-O-Isopropylidene-β-L-idofuranuronic-1,6-13C2 acid γ-lactone
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1,2,3,6-Tetra-O-benzoyl-β-D-mannopyranose
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2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl fluoride
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2-O-(2-Acetamido-2-deoxy-β-D-galactopyranosyl)-D-galactose
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Lactosylceramid
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4-Methylphenyl 4,6-O-benzylidene-1-thio-β-D-galactopyranoside
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4-Methylphenyl 2,3-di-O-benzyl-4,6-O-benzylidene-1-thio-α-D-mannopyranoside
