This kit developed for protein deglycosylation includes our DeGlycoMx, a premixed cocktail of the enzymes required to remove all N-linked oligosaccharides and most O-linked sugars from 0.5 mg of glycoprotein, via 10 reactions of up to 50 micrograms of protein per reaction. The kit is easy-to-use and effective: add 2 µL of the DeGlycoMx enzyme to your denatured sample and the included buffer and incubate for 3 hours at 37°C. The enzymes are provided as one 20 µL premixed cocktail including PNGase F (Elizabethkingia meningosepticum), O-Glycosidase (Streptococcus pneumoniae), Sialidase (Arthrobacter ureafaciens), β-Galactosidase (Streptococcus pneumoniae), Glucosaminidase (Streptococcus pneumoniae). Kit includes enzyme plus reaction buffers. Sufficient for up to 20 reactions.

规格	20 µL premix